[vc_row margin_bottom=”50px”][vc_column][vc_column_text el_class=”pagerbp”]
RNA Silencing Data Package
Using RNA-seq, we provide a comprehensive analysis of the effect of RBP deregulation on gene expression patterns, expression levels and alternative splicing.
[/vc_column_text][/vc_column][/vc_row][vc_row][vc_column width=”1/4″][bsf-info-box icon=”Defaults-flask” icon_size=”32″ icon_color=”#16b718″ title=”Bench work and results” pos=”left”]RT-PCR data for shRNA silencing efficiency in HeLa cells.[/bsf-info-box][/vc_column][vc_column width=”1/4″][bsf-info-box icon=”Defaults-cube” icon_size=”32″ icon_color=”#16b718″ title=”Basic Analysis” pos=”left”]Basic analysis of RNA-seq data, including sequencing quality assessment, clean reads assessment, mapping to human genome, sequencing saturation analysis and gene detection analysis, etc.[/bsf-info-box][/vc_column][vc_column width=”1/4″][bsf-info-box icon=”Defaults-cubes” icon_size=”32″ icon_color=”#16b718″ title=”Advanced Analysis” pos=”left”]Advanced analysis of RNA-seq data, including differentially expressed gene (DEG) analysis, alternative splicing event analysis and gene functional enrichment, etc.[/bsf-info-box][/vc_column][vc_column width=”1/4″][bsf-info-box icon=”Defaults-puzzle-piece” icon_size=”32″ icon_color=”#16b718″ title=”Data” pos=”left”]We provided the original RNA-seq data for two biological replicates and controls at 10G/sample.[/bsf-info-box][/vc_column][/vc_row][vc_row margin_bottom=”30px”][vc_column]
[/vc_column][/vc_row][vc_row type=”1″ margin_top=”30px” margin_bottom=”-50px” css=”.vc_custom_1478226649791{margin-bottom: -50px !important;}”][vc_column][vc_column_text el_class=”pagerbp” css=”.vc_custom_1478594406652{margin-top: 30px !important;}”]Bonus! The package includes the shRNA silencing plasmid for each RBP.
- Provide shRNA target sequences.
- U6 polymerase III promoter for mammalian shRNA expression.
- Kanamycin and Blasticidin resistance markers for easy selection of transformed or transfected cells.
- MMLV LTR sequences for packaging into retroviral particles.
- pCMV-GFP expression to assess transfection efficiency.
- Suitable for creating transient or stable cell lines.
RBP Protein List
[/vc_column_text][wpdatatable id=”12″][/vc_column][/vc_row]