RBP Overexpression Data Package (RNA-Seq)

Using RNA-seq, we provide a comprehensive analysis of the effect of RBP deregulation on gene expression patterns, expression levels and alternative splicing.

[bsf-info-box icon=”Defaults-flask” icon_size=”32″ icon_color=”#16b718″ title=”Bench work and results” pos=”left”]The effect of RBP deregulation in HeLa cells on cell proliferation, migration and apoptosis.[/bsf-info-box]
[bsf-info-box icon=”Defaults-cube” icon_size=”32″ icon_color=”#16b718″ title=”Basic Analysis” pos=”left”]Basic analysis of RNA-seq data, including sequencing quality assessment, clean reads assessment, mapping to human genome, sequencing saturation analysis and gene detection analysis, etc.[/bsf-info-box]
[bsf-info-box icon=”Defaults-cubes” icon_size=”32″ icon_color=”#16b718″ title=”Advanced Analysis” pos=”left”]Advanced analysis of RNA-seq data, including differentially expressed gene (DEG) analysis, alternative splicing event analysis and gene functional enrichment, etc. [/bsf-info-box]
[bsf-info-box icon=”Defaults-puzzle-piece” icon_size=”32″ icon_color=”#16b718″ title=”Data” pos=”left”]We provided the original RNA-seq data for two biological replicates and controls at 10G/sample.[/bsf-info-box]

Bonus! The package includes the overexpression plasmid for each RBP.

  • Provide RBP ORF sequences.
  • CMV promoter for RBP expression, fused to 3x N-terminal FLAG epitope.
  • Dicistronic expression of IRES linked GFP to assess transfection efficiency.
  • Ampicillin resistance marker.
  • Suitable for transient transfection of a variety of mammalian cells. For creating stable cell lines, cotransfection with an additional plasmid that expresses a selectable marker is required.

RBP Protein List